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Product and service portfolio
Vector production
The panel of recombinant virus systems available at VVf includes Lentivirus, Retrovirus, Adenovirus and virus Adeno-associado (AAV).
The service offers second and third generation Lentiviruses derived from HIV skeletons pseudotyped with the vesicular stomatitis virus (VSV) envelope. On request, we can provide Lentivurus pseudotyped with other membrane glycoproteins.
The retroviral vectors we provide are derived from the Moloney Murine Leukemia Virus (Mo-MLV) pseudotyped with anpho-, eco- and xenotropic envelopes. The adenoviral vectors generated include backbones derived from Adenonvirus 5 (Ad5) with deletions in the E1 and E3 regions.
The available AAV vectors are based on the AAV2 system pseudotyped with capsids of different serotypes that have demonstrated their ability to transduce preferably specific tissues with high efficiency: in vitro, muscle, CNS (AAV2); heart, lung (alveoli), liver (AAV9); liver, muscle, eye, CNS (AAV8).
Recombinant vector stocks are delivered as concentrated/purified and titrated batches using methods that allow obtaining consistent values of transducer units/viral particles ratio. The titer by biological measurement (transduction units) is routinely calculated whenever appropriate reporter genes are used. Rapid quantification by qRT-PCR is used to titrate all types of recombinant viruses offered. In the case of Adenoviruses, replication competent adenoviral particles (RCA) are also measured as a biosafety measure.
Vector development
VVf promotes collaborations with other laboratories to facilitate the development of new vectors. The idea is to incorporate and validate relevant innovations in the field and make them accessible to researchers. The service is involved in the design and creation of custom viral vectors that fit the specific needs of researchers.
Prices and estimated times
Our goal is to produce vectors at the lowest possible cost. We operate as a non-profit “core facility” and therefore both the price and any verification test are provided at cost, that is, without charge on what the production costs of the vectors represent as well as those derived from their implementation. ready and maintained at a quality standard, which represents the added value over the production cost.
The prices of the different products and procedures will soon be available in an access link from this page.
Biosecurity
A document will be prepared to optimize the use of vectors in biosafe conditions as well as the GMO and manipulation registration protocol in collaboration with the CNIC Biosafety Service.
