Zeiss LSM 780 Upright confocal - multiphoton

    Multi-channel combined multiphoton-confocal system with dipping objectives and non-descanned detectors for in vivo 3D and 4D imaging. 

    ApplicationsIn vivo confocal and multiphoton imaging, SHG, 2-channel TCSPC FLIM, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation)
    SoftwareZEN 2011 SP7 (black edition) 64bits version 14,0,22,201
    ObjectivesW Plan-Apochromat 20x/1,0 DIC M27 75mm WD 1.8mm dipping
    W Plan-Apochromat 40x/1,0 DIC M27 WD 2.5mm dipping
    Plan-Apochromat 10x/0,45 M27 WD 2mm
    IncubatorLarge size incubator for temperature and CO2 control
    Heatable stage insert
    StagesMotorized
    Sample Setup35 and 60 mm Petri dishes, standard microscope slides
    Illumination SourceCoherent Chameleon Vision-S Laser (pulse 75 fs). 690-1050 nm
    Diode 405 nm
    Argon Ion Laser: 458, 488, 514 nm
    High power diode 561 nm
    Diode 633 nm
    Xcite mercury lamp
    Detectors/Cameras2 PMTs full spectral
    1 spectral PMT detector (GaAsP) with 32 channels
    1 LSM Zeiss BIG1 module with filters: (cyan/yellow), (blue/green), (green/red)
    1 PMT detector for transmitted light
    ConfigurationUpright
    Epi-Filters

    DAPI: Fs49 Ex G365 FT 395 Em BP 445/50

    GFP: Fs 38 Ex BP 470/40 FT 495 Em BP 525/50

    Red: FS 43 Ex BP 550/25 FT 570 Em BP 605/70

    ScannersGalvano
    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes