Nikon ECLIPSE Ti-TimeLapse

    Wide field Fluorescence microscope ideal for in vivo imaging with multiple sample carrier adaptors and flexible to all type of acquisition.

    Inverted wide field Nikon microscope with side mounted camera and LED illumination system
    ApplicationsTransmitted light, wide field fluorescence microscopy, in vivo imaging
    SoftwareNikon AR 4.30.02 (b925) 64bits
    Objectives

    Plan Fluor 4x/0,13 PhL DL
    Plan Fluor 10x/0,3 Ph1 DLL
    S Plan Fluor ELWD 20x/0,45 Ph1 ADM
    Plan Apo λ 20x/0,75
    Plan Fluor ELWD 40x/0.6 Ph2 DM

    Plan Apo 60x/1.2 WI DIC H (under request)

    IncubatorMini-incubator for temperature control and CO2
    StageMotorized stage
    Sample Setup35 mm Petri dishes, standard microscope slides, multiwell plates
    Illumination SourceCool-LED pe-4000, Hallogen lamp
    Detectors/CamerasOrca ER Hamamatsu CCD
    ConfigurationInverted
    Epi- FiltersDAPI: Exc 377-398nm; Dicr409LP; Em 417-477nm (Semrock DAPI-1160A)
    GFP: Exc 457-487nm; Dicr520LP; Em 502-538nm (Semrock GFP-3035B)
    RED: Exc 542-582nm; Dicr593LP; Em 604-644nm (Semrock TxRed-4040B)
    Infrared Cy5: Exc 608-648nm;Dicr660LP; Em 672-712nm (Semrock Cy5-4040C)
    Quadruple: Exc 381-404/461-488/543-566/626-644nm//Dichroics 414-450/500-530/580-611/660-800nm//Em 414-450/500-530/580-611/661-800nm (Semrock LED-DA/FI/TR/Cy5-A-000)