Leica SP8 Navigator with on-the-fly deconvolution [cofounded by FEDER “Una manera de hacer Europa”]

    Confocal microscope for the multidimensional acquisition of samples. With the Lightning and Navigator packages. The first allows the acquisition of higher resolution images by automation of microscope settings for on the fly or subsequent deconvolution of images. The second package allows for easy navigation through samples in diverse sample holders and for the setting and acquisition of complex multidimensional acquisitions.

    ApplicationsConfocal and higher-resolution 3D imaging. Lightning and Navigator packages
    SoftwareLAS X 3.5.7. 23225
    Objectives

    HC PL APO CS2 10x/0.4 Dry
    HC PL Apo CS2 20x/0.75 IMM
    HC PL Apo CS2 40x/1.3 OIL
    HC PL Apo CS2 63x/1.4 OIL
    HC PL Apo CS2 63x/1.3 Glycerol

    HC PL FLUOTAR5x/0.15 Dry (under request)

    IncubatorYes
    StagesMotorized stage
    Sample Setup35 mm Petri dishes, standard microscope slides, culture multiwall plates
    Illumination SourceDiode 405 nm, 442 nm
    White Light Laser: from 470 to 670 nm up to 8 lines simultaneously
    Intensilight fluorescence lamp
     Detectors/Cameras2 PMTs full spectral
    3 Hybrid detectors full spectral
    1 detector for transmitted light
    Configuration Inverted
    Epi-FiltersBlue (A): exc BP 340-380 Dichr400 Em LP 425
    Green (I3): exc BP 450-490 Dichr510 Em LP 515
    Red (N2.1): exc BP 515-560 Dichr580 Em LP 590
    ScannersGalvano/Resonant
    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes