Leica SP5

    Confocal for in-vivo or fixed samples multi-colour imaging. Tile scan and automatic multi-positioning available. Wizard for FRET, FRAP experiments.

    ApplicationsConfocal, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation)
    SoftwareLAS-AF 2.7.3. build 9723
    ObjectivesHCX PL APO CS 10x 0,4 dry
    HCX PL APO lambda blue 20x 0,7 multi-immersion
    HCX PL APO CS 40x 1,25 oil
    HC PL APO 63x 1,2 water
    HCX PL APO CS 63 x 1,3 Glyc
    HCX PL APO lambda blue 63x 1,40 oil
    Also under previous request:
         HC PL FLUOTAR 20x/0.50 PH2
         HCX PL FLUOTAR 5x/0.15
    IncubatorLarge size incubator for temperature and CO2 control
    StageMotorized stage
    Sample Setup35 mm Petri dishes and standard microscope slides
    Illumination SourceDiode 405 nm
    Argon Ion Laser: 458,476,488, 496, 514 nm
    Diode 561 nm
    HeNe 594 nm
    HeNe 633 nm
    Intensilight fluorescence lamp
    Detectors/Cameras4 spectral PMT detectors
    1 spectral Hybrid Detector
    1 PMT for transmitted light
    ConfigurationInverted
    Epi- FiltersDAPI (A): exc BP 340-380 Dichr400 Em LP 425
    Green (I3): exc BP 450-490 Dichr510 Em LP 515
    Green Restrictive (L5): exc BP 460-500 Dichr505 Em BP 512-542
    Red (N2.1): exc BP 515-560 Dichr580 Em LP 590
    ScannerGalvano/Resonant