Leica SP5
Confocal for in-vivo or fixed samples multi-colour imaging. Tile scan and automatic multi-positioning available. Wizard for FRET, FRAP experiments.
![](https://www.cnic.es/sites/default/files/sp5_dos_pantallas_opt_0.jpg)
Applications | Confocal, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation) |
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Software | LAS-AF 2.7.3. build 9723 |
Objectives | HCX PL APO CS 10x 0,4 dry HCX PL APO lambda blue 20x 0,7 multi-immersion HCX PL APO CS 40x 1,25 oil HC PL APO 63x 1,2 water HCX PL APO CS 63 x 1,3 Glyc HCX PL APO lambda blue 63x 1,40 oil Also under previous request: HC PL FLUOTAR 20x/0.50 PH2 HCX PL FLUOTAR 5x/0.15 |
Incubator | Large size incubator for temperature and CO2 control |
Stage | Motorized stage |
Sample Setup | 35 mm Petri dishes and standard microscope slides |
Illumination Source | Diode 405 nm Argon Ion Laser: 458,476,488, 496, 514 nm Diode 561 nm HeNe 594 nm HeNe 633 nm Intensilight fluorescence lamp |
Detectors/Cameras | 4 spectral PMT detectors 1 spectral Hybrid Detector 1 PMT for transmitted light |
Configuration | Inverted |
Epi- Filters | DAPI (A): exc BP 340-380 Dichr400 Em LP 425 Green (I3): exc BP 450-490 Dichr510 Em LP 515 Green Restrictive (L5): exc BP 460-500 Dichr505 Em BP 512-542 Red (N2.1): exc BP 515-560 Dichr580 Em LP 590 |
Scanner | Galvano/Resonant |