Leica 4-Color dSTORM (Ground-State Depletion Single Molecule Localization)
Based on standard immunostaining techniques it is a flexible system that combines TIRF and epifluorescence with single molecule localization imaging achieving a lateral resolution of up to 20 nm. Customized for N&B application on live cells.
![](https://www.cnic.es/sites/default/files/storm_1.jpg)
Applications | dSTORM/GSD (Ground-State Depletion), TIRF, N&B, wide field microscopy |
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Software | LAS X 1.1.0.12420 |
Objectives | HCX PL APO 160x NA 1.43 Oil HCX PL APO 20x NA 0.7 Dry |
Incubator | Large size incubator for temperature and CO2 control |
Stages | Manual in XY, Motorized stage in Z axis |
Sample Setup | 35 mm Petri dishes and standard microscope slides |
Illumination Source | High power solid state diode lasers: 405 nm (30mW) 488 nm (300mW) 532 nm (500mW) 642 nm (500mW) Intensilight fluorescence lamp |
Detectors/Cameras | EMCCD back-illuminated camera ANDOR iXON Ultra DU897 |
Configuration | Inverted |
Filters | QGS: Blue, Green, Red, FarRed: Exc: 400-410; 483-493; 527-537; 637-647. Dichroic: 417; 496; 544; 655. Em: 421-477; 497-519; 547-621; 666-732. |
External Access | https://www.cnic.es/en/investigacion/2/1187/interes |