ISS ALBA-FLIM Nikon ECLIPSE Inverted multiphoton [cofounded by FEDER “Una manera de hacer Europa”]

    Two-channel multiphoton system for in vivo FLIM and N&B

    ApplicationsFluorescence LifeTime Imaging (FLIM), N&B
    SoftwareVistaVision ISS 4.2
    Objectives

    Plan Apo VC 60x/1.2 WI

    Plan Apo VC 60x/1.4 Oil

    S Fluor 40X/1.3 Oil DIC H/N2

    40x/0.9  DIC Dry

    S Fluor 20x /0.75 Dry

    10x/0.45 DIC N1

    IncubatorMini-incubator for temperature control and CO2
    StageX,Y,Z motorized stage
    Sample Setup35 mm Petri dishes and standard microscope slides
    Illumination SourceSpectra-Physics MaiTai tunable DS Laser. 690-1040 nm (pulse <100ps)
    Detectors/Cameras

    2 PMTs

    2 APDs

    2 Hybrid PMT detectors

    Configuration Inverted NIKON ECLIPSE
    Epi-FiltersDAPI, TRTC, GFP
    ScannersGalvano
    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes