4D Customized SPIM

    Selective Plane Illumination customized system with optical sectioning capacity for multicolour acquisition of cleared thick samples, small organs, tissues and whole embryos. Information from different views can be acquired to obtain a 3D reconstruction of the specimen.

    The custom made selective plane illumination microscope with a temperature chamber
    ApplicationsMulti-color plane illumination microscopy (SPIM) on thick samples
    SoftwareOpt3D (Customized software developed by 4D-Nature Imaging Consulting, S.L.)
    ObjectivesMitutoyo M plan Apo 2x 0.055 DRY
    Mitutoyo M plan Apo 5x 0.14 DRY
    Mitutoyo M plan Apo 10x 0.28 DRY
    IncubatorLarge size incubator for temperature and CO2 control
    StagesMotorized arm movement in XYZ
    Sample SetupSyringe, capillary tube, glass tube, quartz cuvette
    Illumination SourceDiode 405 nm
    Diode 488nm
    Diode 532nm
    Diode 638nm
     Detectors/Cameras ORCA-Flash sCMOS Camera (Hamamatsu)
    Configuration Special holder for big specimens
    FiltersDAPI Em: 417-477nm
    CFP Em: 467-497nm
    GFP Em: 500-520nm; 502.5-547.5nm
    RED Em: 558-586nm; 605-625nm; 612-644nm
    FAR RED Em: 661.5-690.5nm