Advanced Multi-modal Nanoscopy Biomedical Platform

CNIC Advanced Multi-modal Nanoscopy Biomedical Platform

Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia. Subprograma Estatal de Infraestructuras Científicas y Técnicas, y Equipamiento

The CNIC Advanced Multi-modal Nanoscopy Biomedical Platform is a super-resolution optical/fluorescence imaging system with unique features. The platform is based on an innovative gated STED-3D SP8 microscope equipped with a super-continuum excitation laser and two stimulating lasers (592 and 660 nm) for super-resolved colocalization imaging. High sensitivity is ensured by two hybrid detectors. The platform is completed with a Ground State Depletion-Total Internal Reflection microscope for single molecule localization imaging. This second system has been customized for imaging of fluorescence fluctuations by Number of Molecules and Brightness analysis in live cells. The platform allows a comprehensive approach to super-resolution imaging using a variety of fluorescent probes, ranging from spectral variants of fluorescent protein to standard chemical dyes.

Project title Plataforma Biomédica Avanzada CNIC en Nanoscopía Multimodal
Expedient IPT-2011-1307-010000
Funds Raised 900.000 euros
Project PI Dr. Miguel Torres Sánchez
Plataform Coordinator Dr. Valeria R. Caiolfa
Funding Agency Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016. Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia, cofinanciación con fondos FEDER (Resolución Definitiva 10/12/14)