3D AVC explant of E9.5 WT embryo

    3D AVC explant of E9.5 WT embryo. GFP expression of mTmG transgenic mice upon VECadherinCREERT2 activation
    using a low dosage pulse of Tamoxifen. GFP is expressed only in a small number of endocardial cells.
    Confocal Time Lapse movies showed transformed cells in green building cellular projections toward collagen matrix.

     

    2D ventricular explant of WT E9.5 embryo

    2D ventricular explant of WT E9.5 embryo. GFP expression of mTmG transgenic mice upon VECadherinCREERT2
    activation using a low dosage pulse of Tamoxifen. GFP is expressed only in a small number of endocardial cells.
    Confocal Time Lapsemovies showed single endocardial cells forming lamellipodia in the growth
    front, and cells forming filopodia when surrounded by other endocardial cells.

     

    3D reconstruction of an E10.5 mouse heart stained with SMA, IB4 and DAPI

    3D reconstruction of an E10.5 mouse heart stained with SMA, IB4 and DAPI to show the complex structure of the trabeculated ventricle.

     

    Group picture

    Group picture