Leica 3-Colour Fast TIRF AM
TIRF (Total Internal Reflection Microscopy) illumination system for selective visualization of close to substrate structures such as the basal plasma membrane, and studies of cell motility, protein aggregation, vesicle traffic, etc. Resolution in Z direction reaches up to 70 nm. Customized for N&B application on live cells.
![](https://www.cnic.es/sites/default/files/tirf_4.jpg)
Applications | TIRF, wide field and N&B Microscopy |
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Software | LAS-AF 2.6.0. build 7266 |
Objectives | HCX PL APO 20x 0,7 dry HCX PL APO 40x 1,25 oil HCX PL APO 100x 1,46 oil |
Incubator | Large size incubator for temperature and CO2 control. Pecon Heatable Stage Insert. |
Stages | Motorized |
Sample Setup | 35mm Petri dishes and standard microscope slides |
Illumination Source | Laser Diodes Lines: 488, 564, 633 nm Intensilight fluorescence lamp |
Detectors/Cameras | Andor DU 897 EMCCD Andor DU 8285 VP EMCCD |
Configuration | Inverted |
Filters | GFP: BP 490/20 (500) BP 525/50 RFP: BP 552/24 (570) BP 605/65 Cy5: BP 635/10 (650) BP 720/60 VBG: BP 490/20 422/44 552/24 (435/505/550) BP 465/45 545/55 610/65 |
External Access | https://www.cnic.es/en/investigacion/2/1187/interes |