Leica gated STED-3X- WLL SP8 [cofounded by FEDER “Una manera de hacer Europa”]
STED super resolved imaging and co-localization in 3D. Optimal for fixed samples. Gating, photon counting detectors and super-continuum laser (WLL) provide high flexibility and sensitivity also in the confocal mode. Also with the Navigator package for easy navigation through samples in diverse sample holders and for the setting and acquisition of complex multidimensional acquisitions..
![](https://www.cnic.es/sites/default/files/sted.jpg)
Applications | Confocal and super-resolution 3D STED imaging, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation). Navigator package. |
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Software | LAS X 3.5.7. 23225 |
Objectives | HC PL APO 10x/0.40 CS2. Dry HC PL Apo CS2 20x/0.75 IMM HC PL Apo CS2 40x/1.3 OIL HC PL Apo CS2 63x/1.4 OIL HC PL Apo CS2 93x/1.3 GLY (Under request) HC PL Apo CS2 100x/1.4 OIL |
Incubator | Yes |
Stages | Motorized stage |
Sample Setup | 35 mm Petri dishes and standard microscope slides |
Illumination Source | Diode 405 nm White Light Laser: from 470 to 670 nm up to 8 lines simultaneously STED Depletion Laser lines: 592nm, 660nm, 775 Intensilight fluorescence lamp |
Detectors/Cameras | 2 PMTs full spectral 2 Hybrid detectors full spectral 1 detector for transmitted light |
Configuration | Inverted |
Epi- Filters | Blue (A): exc BP 340-380 Dichr400 Em LP 425 Green (I3): exc BP 450-490 Dichr510 Em LP 515 Red (N2.1): exc BP 515-560 Dichr580 Em LP 590 |
Scanners | Galvano/Resonant |
External Access | https://www.cnic.es/en/investigacion/2/1187/interes |